Fig. 10

Crenigacestat inactivates iCCA hCAFs. Crenigacestat downregulates α-SMA (green signal, Panel A) and Vimentin (green signal, Panel B) expression in treated iCCA hCAFs, as detected by immunofluorescence staining. For both protein markers, staining quantification was calculated on nuclei number as the mean of five images per cell slide treated with increasing Crenigacestat concentrations compared to untreated. The scale bar represents 10 μm, magnification 20x. Panel C. Vimentin (green) and α-SMA (red) in overlapping stains (yellow) were co-immunolocalized in the same primary cell cultures. The scale bar represents 50 μm, magnification 20x.*p < 0.05, **p < 0.001, ***p < 0.0001 calculated with Student’s t-test