Fig. 4

Crenigacestat inhibits the TGF-β pathway in iCCA PDX models. Panel A. Real-time PCR for TGFB1 on PDX mice reveals a reduction of TGF-β gene expression in PDX mice treated with Crenigacestat. Panel B and C. Crenigacestat reduces the TGF-β/Smad pathway in PDX-treated mice, as detected by Western blot analysis and immunofluorescence staining. The scale bar represents 50 μm. ***p < 0.0001. Magnification 20X