Fig. 2

MK5-AS1 regulated CRC invasion and migration in vitro and vivo. a Transwell assays were used to determine the invasion and migration abilities of HCT116 and SW620 cells after MK5-AS1 overexpression and knockdown. Scale bar, 100 μm for A. b Immunoblotting of EMT-related markers after transfection in CRC cells. c Nude mice were injected with HCT116 cell after MK5-AS1 knockdown into tail vein. The number of metastatic nodules of lung was shown and counted. The black arrow marked metastatic nodules. d Tumor progression was monitored using a small animal imaging system, HE-stained lung sections (Scale bar, 100 μm) and antibody vimentin of metastatic nodules were shown (× 400 magnification). The black arrow marked metastatic nodules. e The Ensembl Genome browser (http://asia.ensembl.org/) showed that MK5 was the nearby gene of MK5-AS1. f Immunoblotting was used to investigate the level of MK5 of HCT116 and SW620 cells after intervening MK5-AS1, respectively. The data represented the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001