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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Correction: Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge

Fig. 7

MK5-AS1/let-7f-1-3p/SNAI1 ceRNA network. a Transwell assays were used to perform the invasion and migration abilities in HCT116 and SW620 cells after transfection. Scale bar, 100 μm for A. b The potential binding sites among MK5-AS1, let-7f-1-3p and SNAI1. c Upper panel, the potential binding sites between let-7f-1-3p and SNAI1. Lower panel, the luciferase reporter plasmids containing wild type (WT) or mutant (MUT) SNAI1 3′ UTR were cotransfected into HCT116 cells with let-7f-1-3p. d MK5, c-Jun, p-c-Jun(S63) and SNAI1 expressions were detected in HCT116 and SW620 cells by immunoblotting after transfection with let-7f-1-3p inhibitor or let-7f-1-3p mimics. e Left panel, the effects of si-MK5-AS1, let-7f-1-3p inhibitor and si-MK5-AS1 + let-7f-1-3p inhibitor on protein levels of SNAI1 in HCT116 and SW620 cells. Right panel, the effects of pcDNA3.1 MK5-AS1, let-7f-1-3p mimics, and pcDNA3.1 MK5-AS1 + let-7f-1-3p mimics on protein levels of SNAI1 in HCT116 and SW620 cells. Data were shown as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001

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