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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: A functional loop between YTH domain family protein YTHDF3 mediated m6A modification and phosphofructokinase PFKL in glycolysis of hepatocellular carcinoma

Fig. 2

YTHDF3 overexpression promotes proliferation, migration and invasion of Sk-Hep-1 and HepG2 cells in vitro and tumor growth and lung metastasis of Sk-Hep-1 in vivo.a-b CCK8 proliferation assays and colony formation assays were performed to determine cell proliferation of Sk-Hep-1 and HepG2 after YTHDF3 overexpression. c-d Transwell migration assays and wound healing assays were performed to determine cell migration of Sk-Hep-1 and HepG2 after YTHDF3 overexpression. The Image J software was used to quantified cells’ migration ability. Scale bar 200 μm. e Transwell invasion assays were performed to determine cell invasion of Sk-Hep-1 and HepG2 after YTHDF3 overexpression. The Image J software was used to quantified cells’ invasion ability. Scale bar 200 μm. f Representative images of tumor growth in xenografted BALB/c nude mice with Sk-Hep-1 overexpression YTHDF3 cells (left). The growth curves and the average weight of xenograft tumors were shown (right). g Representative images of HE and IHC of YTHDF3 and Ki67 of xenograft tumor (left), IHC scores of YTHDF3 and Ki67 were shown in bar graphs (right). Scale bar 200 μm. h Representative images of HE staining of orthotopic lung metastasis model (upper). The number and total area of metastatic foci were shown in bar graphs (lower). Scale bar 5 mm. Data are presented as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001). Arrows indicate the location of positive signal of YTHDF3 or Ki67 protein

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