Fig. 3

Reduced expression of ELF3-AS1 and ELF3 correlates with poor prognosis in GC. A ELF3 and ELF3-AS1 were EMT-related genes that highly co-expressed with epithelial biomarkers in GC. B The ELF3-AS1 expression in 30 paired of GC tissues and adjacent non-tumor tissues was examined by qRT-PCR. C, D The ELF3-AS1 expression in GC patients with different histopathological types and differentiation degrees. E Overall Survival analysis using GEPIA web tool showed that ELF3-AS1 predicts favorable prognosis in GC. F The ELF3 expression level in 30 paired of GC tissues and adjacent non-tumor tissues was examined by qRT-PCR. G Representative IHC images on the tissue microarray (TMA) probed with the anti-ELF3 antibody (scale bars=100 μm or 25 μm, respectively) are shown. H The difference in expression levels of ELF3 between intestinal GC tissues and diffuse GC tissues. I, J ELF3 expression level in different T-stages and Borrmann-stages of GC tissues from the GSE62254 cohort. K Kaplan-Meier survival curves of overall survival and disease-free survival based on ELF3 expression in the GC cohort GSE62254. **, P < 0.01. ***, P < 0.001. ****, P < 0.0001