Fig. 8
The ILF2/ILF3 interacts with ELF3-AS1/ELF3 RNA duplex to affect RNA duplex stability. A The gene structure and location of ELF3-AS1 and ELF3 genes. B The normalized expression (FPKM value) of different ELF3 transcripts after knockdown of ELF3-AS1 was shown in the plot. Knockdown of ELF3-AS1 remarkably decreased the expression of ELF3-201 transcripts in GC cell lines. C, D RIP-qPCR assay verified the interaction between ELF3-201 transcripts and ILF2/ILF3 complex. E The transcripts abundance of ELF3 and ELF3-AS1 in ILF3-silenced samples was detected by RNA-seq. (F, G) The transcripts abundance of ELF3 and ELF3-AS1 in ILF2-silenced samples was detected by RNA-seq. (H) ILF2 knockdown affected the alternative splicing of the ILF3 gene. **, P < 0.01