Fig. 6

MDHDH suppresses the growth of GBM in vitro and in vivo via PSMA1. A U87 and U251 cells were transfected with the indicated plasmid constructs, and GBM cell line viability was examined by CCK-8 assay (left panel: U87 cell line, right panel: U251 cell line). B U87 and U251 cell (NC, OE MDHDH and OE MDHDH+siPSMA1) proliferation determined by EdU staining assay. C Migration and invasion of the transfected U87 and U251 cell lines (NC, OE MDHDH and OE MDHDH+siPSMA1) determined by transwell assay. D In vivo luminescent imaging of in situ tumor-bearing nude mice at the indicated time points. The total flux of the nude mice was counted 7 days and 14 days after intracranial implantation of luciferase-tagged U87 cells transfected with MDHDH, MDHDH+shPSMA1 or control plasmids by IVIS spectrum. The survival curves of the nude mice with xenografted tumors intracranially were recorded for 15 days. E Immunohistochemistry of Ki67 and MDH2 comparing NC, OE MDHDH or OE MDHDH+shPSMA1 (upper). Ki67 and MDH2 H-scores of different xenograft tissues (bottom). F Representative micrographs of HE-stained sections of mouse brain tissues under low- (scale bar = 250 μm) and high-power (scale bar = 200 μm) magnification 15 days after intracranial implantation of U87 cells infected with a lentiviral vector expressing NC, OE MDHDH or OE MDHDH+shPSMA1