Fig. 2

Silencing of CEBPA-DT represses tumor growth and metastasis of hepatoma cells in vitro. A Cell viability of indicated HCCLM9 and SNU-449 cells were measured by CCK-8 assays. B Cell proliferation of indicated HCCLM9 and SNU-449 cells were measured by EdU assays. Scale bar, 200 μm. C-D Cell migration and invasion capacity of indicated HCCLM9 and SNU-449 cells were measured by transwell migration and matrigel invasion assays. Scale bar, 100 μm. E–F Cell migration capacity of indicated HCCLM9 and SNU-449 cells were measured by scratch wound-healing assays. Scale bar, 100 μm. G-H Cell apoptosis rates in indicated HCCLM9 and SNU-449 cells were measured by flow cytometry. I-J Cell-cycle distribution of indicated HCCLM9 and SNU-449 cells were measured by propidium iodide staining followed by flow cytometry. Date are presented as mean ± SD; n = 3. Student’s t test was used. *p < 0.05, **p < 0.01, ***p < 0.001