Fig. 3

CEBPA-DT promotes tumor growth and metastasis of hepatoma cells in vitro. A Cell viability of indicated Hep3B and Huh7 cells were measured by CCK-8 assays. B Cell proliferation of indicated Hep3B and Huh7 cells were measured by EdU assays. Scale bar, 200 μm. C-D Cell migration and invasion capacity of indicated Hep3B and Huh7 cells were measured by transwell migration and matrigel invasion assays. Scale bar, 100 μm. E–F Cell migration capacity of indicated Hep3B and Huh7 cells were measured by scratch wound-healing assays. Scale bar, 100 μm. G-H Cell apoptosis rates in indicated Hep3B and Huh7 cells were measured by flow cytometry. I-J Cell-cycle distribution of indicated Hep3B and Huh7 cells were measured by propidium iodide staining followed by flow cytometry. Date are presented as mean ± SD; n = 3. Student’s t test was used. *p < 0.05, **p < 0.01, ***p < 0.001