Fig. 4

CEBPA-DT promotes tumor growth and metastasis in vivo. A-B Tumor volume and weight of subcutaneous xenografts in nude mice injected with indicated HCCLM9 and SK-Hep1 cells. C-D Representative images (fluorescence) of intrahepatic metastatic nodules in liver orthotopic-implantation tumor models with indicated HCCLM9 and SK-Hep1 cells. E–F Representative microscopic views of HE staining and the corresponding statistical analyses of intrahepatic metastatic nodules (black arrows) in liver orthotopic-implantation tumor models injected with indicated HCCLM9 and SK-Hep1 cells. Scale bar, 50 μm. G-H Representative bioluminescent images and the corresponding statistical analyses of bioluminescent tracking plots for lungs of nude mice injected with indicated HCCLM9 and SK-Hep1 cells through tail vein. I-J Representative microscopic views of HE staining and the corresponding statistical analyses of lung metastatic nodules (black arrows) in nude mice injected with indicated HCCLM9 and SK-Hep1 cells through tail vein. Scale bar, 50 μm. Date are presented as mean ± SD; n = 3. Student’s t test was used. *p < 0.05, **p < 0.01, ***p < 0.001