Fig. 6

CEBPA-DT promotes the metastasis of hepatoma cells through the hnRNPC-DDR2 axis. A Enrichment analysis showed significantly enriched pathways annotated by the Reactome terms ranked based on the significance of the enrichments. B Expression levels of 8 down-regulated extracellular matrix-related mRNAs screened by enrichment analysis were measured with RT-qPCR. C-D hnRNPC and DDR2 expression levels were detected by western blot and RT-qPCR in hnRNPC silencing cells, respectively. E RIP-qPCR analysis of the enrichment of DDR2 mRNA on hnRNPC relative to IgG in indicated control and CEBPA-DT overexpression cells. F Western blot analysis of DDR2 expression levels in Hep3B and Huh7 cells co-transfected with CEBPA-DT overexpression vectors, hnRNPC siRNAs or the control vectors. G 48 h after transfected with CEBPA-DT overexpression vectors with or without hnRNPC siRNA, DDR2 mRNA levels were detected at different periods after treated with actinomycin D. H Representative bioluminescent images and the corresponding statistical analyses of bioluminescent tracking plots for lungs of nude mice injected with stable CEBPA-DT overexpressing SK-Hep1 cells with or without stable DDR2 silencing. I IHC staining of DDR2. Left: Representative samples of HCC tissues and adjacent non-tumor tissues. Right: IHC scores of 50 HCC tissues and adjacent non-tumor tissues presented as mean ± SEM, Wilcoxon signed-rank test was used. J The correlation between the relative expression of CEBPA-DT and the IHC staining of DDR2 in 50 HCC tissues. The correlation was measured by Pearson correlation analysis. Date are presented as mean ± SD; n = 3. Student’s t test was used. *p < 0.05, **p < 0.01, ***p < 0.001