Fig. 2

CircRUNX1 knockdown suppresses ESCC progression. A and B Expression levels of circRUNX1 (A) and RUNX1 (B) post transfection of three siRNAs targeting circRUNX1. C Colony formation assays were performed in ESCC cell lines with circRUNX1 knockdown. D The cell viability of KYSE150 and TE1 cells were evaluated by CCK-8 assays. E Changes of cell proliferation after knocking down circRUNX1 were detected by EDU assay (Magnification × 100. Scale bar: 100 μm). F Migration and invasion abilities of KYSE150 and TE1 cells after circRUNX1 inhibition were evaluated by transwell assays. G Migration abilities of KYSE150 and TE1 cells after depletion of circRUNX1 were evaluated by wound healing assay. **P < 0.01, ***P < 0.001