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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: IGF2BP2-induced circRUNX1 facilitates the growth and metastasis of esophageal squamous cell carcinoma through miR-449b-5p/FOXP3 axis

Fig. 4

CircRUNX1 serves as miR-449b-5p sponge to promote ESCC progression. A Venn diagram showing the candidate miRNAs predicted to be the binding targets of circRUNX1 and FOXP3 by circBank (http://www.circbank.cn) and Targetscan (http://www.targetscan.org). B Relative expression of 14 candidate miRNAs after knocking down circRUNX1. C Relative expression of miR-449b-5p in KYSE150 and TE1 cells stably expressing circRUNX1. D The luciferase activity was determined in 293 T cells co-transfected with miR-449b-5p mimics/miR-NC and circRUNX1-WT/circRUNX1-MUT. E Anti-Ago2 RIP assays were performed in KYSE150 and TE1 cells to detect the enrichment ability of Ago2 to circRUNX1 and miR-449b-5p. F The correlation between miR-449b-5p and circRUNX1 expression in 41 primary ESCC tissues. G Relative expression of miR-449b-5p in a panel of ESCC cell lines and normal esophageal epithelial cells (HEEC). H-J CCK-8 (H), colony formation (I) and EdU assays (J) were performed to measure the proliferative abilities of TE1 cells transfected with circRUNX1 or circRUNX1 plus miR-449b-5p mimics. K-M Wound healing and transwell assays were utilized to evaluate the migration and invasion capacities of TE1 cells transfected with indicated vectors. Data are represented as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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