Fig. 5

CircRUNX1/miR-449b-5p/FOXP3 axis facilitates ESCC proliferation and metastasis. A Relative expression of FOXP3 in KYSE150 cells and TE1 cells transfected with miR-449b-5p mimics or miR-NC. B Western blot analysis of FOXP3 level in ESCC cells after transfecting miR-449b-5p mimics or miR-NC. C The luciferase activity was determined in 293 T cells co-transfected with miR-449b-5p mimics/miR-NC and FOXP3-WT/FOXP3-MUT. D-F CCK-8 (D), colony formation (E) and EdU assays (F) were performed to measure the proliferative effects of miR-449b-5p and FOXP3 on KYSE150 cells. G-I The migratory or invasion properties of the miR-449b-5p inhibition with or without FOXP3 depletion in KYSE150 cells and control cells were analyzed by wound healing and transwell experiments. Data are represented as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001