Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: IGF2BP2-induced circRUNX1 facilitates the growth and metastasis of esophageal squamous cell carcinoma through miR-449b-5p/FOXP3 axis

Fig. 6

IGF2BP2 silencing retards ESCC progression via accelerating circRUNX1 degradation. A Venn diagram showing the candidate RNA binding proteins predicted to combine with circRUNX1by circAtlas (http://circatlas.biols.ac.cn) and RBPmap (http://rbpmap.technion.ac.il). B IGF2BP2 mRNA levels were detected by qRT-PCR in 41 pairs of ESCC tissues and their corresponding normal tissues. C Expression and scoring of IGF2BP2 protein were detected by IHC in 30 pairs of ESCC tissues. D IGF2BP2 immunostaining scores were detected by IHC in ESCC tissues with lymph node metastasis (n = 13) and non-metastasis (n = 17). E and F Changes of circRUNX1 and IGF2BP2 levels were determined in KYSE150 cells upon silencing or overexpression of IGF2BP2. G The correction between circRUNX1 and IGF2BP2 were examined in 41 ESCC tissues. H IHC results showing that higher circRUNX1 expression was related to higher abundance of IGF2BP2 protein in ESCC tissues. I qRT-PCR analysis showing circRUNX1 expression in KYSE150 cells treated with actinomycin D for specific time points. J RIP assay using anti-IGF2BP2 antibody and IgG antibody was executed in KYSE150 cells with or without overexpressing IGF2BP2. K-M CCK-8 (K), colony formation (L) and EdU assays (M) were conducted to investigate the proliferative abilities of KYSE150 cells with IGF2BP2 overexpression or circRUNX1 inhibition. N-P Wound healing and transwell assays showing the migration and invasion capabilities of KYSE150 cells with IGF2BP2 overexpression or circRUNX1 inhibition. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page