Fig. 7

CircRUNX1 targets FOXP3 to accelerate tumorigenesis and metastasis in vivo. A and B The process of constructing subcutaneous tumor models (A) and pulmonary metastasis models (B) in nude mice. C Tumor volumes of indicated groups were measured every 5 days. D Image of subcutaneous tumors from three groups were displayed. E The weights of subcutaneous tumors were quantified. F Representative IHC staining images and quantification of FOXP3 and Ki-67 positive cells in the indicated xenograft tumors. Magnification × 100. G FOXP3 mRNA levels of subcutaneous tumor tissues were detected by qRT-PCR. H The images of lung metastases and statistical analysis of metastatic lung foci. I Representative images from H&E staining of the lung metastasis nodules. *P < 0.05, **P < 0.01, ***P < 0.001