Fig. 2

NAT10 promotes CRC cell proliferation, migration and invasion in vitro. A and B Transfection efficiency of NAT10 in SW480 and HT-29 cells, detected by qRT-PCR, WB, and dot blot. C and D CCK-8 assays were applied to determine the growth curves of NAT10 knockdown or overexpression cells. E and F Colony formation assays were carried out to detect the proliferation of CRC cells. G and H EdU assays were performed to evaluate the cell proliferation ability. I and J The distribution of the cell cycle was detected by flow cytometry in NAT10 knockdown or overexpression cells. K and L Cells were treated with the serum-free medium for 36 h. Flow cytometry was used to detect the apoptotic rates (LR + UR) of cells. M and N Transwell and wound healing assays were used to detect the migration and invasion of CRC cells. LR, early apoptotic cells; UR, terminal apoptotic cells. Data are shown as mean ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns. not significant