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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Stroma-derived miR-214 coordinates tumor dissemination

Fig. 2

Overexpression of miR-214 in stroma cells favors metastasis formation of melanoma and breast cancer cells. A Analyses of experimental lung metastasis formation 8 days after tail vein injection of B16-F10 cells into miR-214wt and miR-214over syngeneic mice. Representative pictures of the whole lungs (a, b; scale bar = 2 mm), arrows indicate metastasis formations. Relative lung metastases between the two groups of mice is shown in the graph for the indicated number (n) of animals. B CMRA-labeled B16-F10 cells (red) were injected into the tail vein of miR-214wt and miR-214over syngeneic mice and extravasation was evaluated 2 h (a, b, c) or 48 h (d, e, f) later. Representative fields of murine lung sections stained for CD31 (green) to highlight blood vessels and counterstained with DAPI (blue); scale bar: 25 µm (a, d). Representative pictures of whole lungs containing red-labeled B16-F10 cells are shown; scale bar: 1 mm (b, c, e, f). Relative extravasated cells in the whole lungs at 48 h is shown in the graph for the indicated number (n) of mice, as mean ± SEM. C-D In vivo tumor growth and metastatic dissemination of B16-F10 (C) or EO771 (D) cells injected subcutaneously in miR-214wt and miR-214.over syngeneic mice, 45 or 30 days pos-inoculation respectively. In (C) tumors were removed 15 days post-injection and analyzed. Relative primary tumor weight and Circulating Tumor Cells (CTCs) are shown in the graphs as mean ± SEM, for the indicated number of mice (n). Two independent experiments were performed and representative results are shown. CMRA = CellTracker™ Orange; CD31 = cluster of differentiation 31; n = number of mice; DAPI = 4’, 6-diamidino-2-fenylindole SEM = standard error of mean. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001

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