Fig. 5

Ablation of miR-214 in stroma cells inhibits metastasis formation of melanoma and breast cancer cells. A-B In vivo tumor growth and metastatic dissemination of B16-F10 (A) and EO771 (B) cells injected subcutaneously in miR-214^wt and miR-214^ko syngeneic mice, respectively 45 or 30 days post-inoculation. In (A) tumors were removed 15 days post-injection. Relative primary tumor weight (measured in grams) and number of Circulating Tumor Cells (CTCs) is shown in the graphs as mean ± SEM, for the indicated number of mice (n). C-D miR-214 expression levels assessed in B16-F10- and EO771-derived xenografts grown in miR-214^wt and miR-214^ko mice by qRT-PCR analysis for the indicated number (n) of animals. E GFP⁺ B16-F10 cells were subcutaneously injected into miR-214^wt and miR-214^ko syngenic mice to generate tumors. 15 days later, mice were sacrificed, transplants dissected and tumor/stroma cells separated by FACS sorting. miR-214 expression levels were measured in GFP⁺ B16-F10 cells before injection (culture) or FACS sorted from subcutaneous tumors (sorted) by qRT-PCR analysis, for the indicated number (n) of animals. C-E Results are shown as fold changes (mean ± SD of triplicates) relative to the median, normalized on U6. E Results are pools of 3 animals per group. n = number of mice. SD = standard deviation. SEM = standard error of mean. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001