Fig. 5

Schwann cells promoted the proliferation and metastasis of SW480 cells. A. The proliferation of SW480 cells co-cultured with Schwann cells or not was assessed via CCK8 for 3 days. B. The proliferation of SW480 cells co-cultured with Schwann cells or not was assessed via colony formation assay for 8 days. C. The expression of p27kip1, CyclinD1, and CyclinE of SW480 cells co-cultured with Schwann cells or not was detected by Western blot and qRT-PCR. D. EDU assay showed that co-cultured with Schwann cells increased the proportion of EDU-positive cells in SW480 cells. Magnification, × 200. E. The wound healing assays showed that co-cultured with Schwann cells significantly augmented the migrative ability of SW480 cells. F. The transwell and tumor invasion assays indicated that co-cultured with Schwann cells increased the migrative and invasive abilities of SW480 cells. G. The Western blot and qRT-PCR assays showed that co-cultured with Schwann cells increased the expression of mesenchymal markers (ZEB1, Vimentin, and N-cadherin), and decreased the expression of epithelial markers, such as E-cadherin and ZO1. H. Immunofluorescence assay indicated that co-cultured with Schwann cells increased mesenchymal markers of SW480cells but reduced epithelial markers. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ***P < 0.001