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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: SRSF10 stabilizes CDC25A by triggering exon 6 skipping to promote hepatocarcinogenesis

Fig. 3

Abundant CDC25A(â–³E6) in HCC results in more malignant phenotypes than the CDC25A(L) isoform. A Expression of the CDC25A long and â–³E6 isoforms in HCC and adjacent nontumor tissue (10 pairs). The relative quantifications of CDC25A(L) and CDC25A(â–³E6) in each pair were shown underneath. B, C, D, E Restorative cell model validation of individual CDC25A(L) or CDC25A(â–³E6) following endogenous CDC25A deprivation by semi-RT‒PCR using three primer sets (B, C, D) and western blotting (E). F, G, H, I CCK-8, EdU, transwell assays, and flow cytometry showed the significantly higher promoting role of CDC25A(â–³E6) in cell viability (F), proliferation (G), invasion (H), and cell cycle (I) than CDC25A(L). Data are presented as the mean ± SD value of three biological replicates. ***p < 0.001 vs. NC, △△p < 0.01 and △△△p < 0.001 vs. CDC25A(L), t test. See representative images in Supplementary Fig. 7

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