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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The traditional chinese medicine monomer Ailanthone improves the therapeutic efficacy of anti-PD-L1 in melanoma cells by targeting c-Jun

Fig. 4

Improved antitumor effect of anti-PD-L1 mAb and AIL cotreatment in B16-F10 tumor-bearing C57BL/6 mice. A-C C57BL/6 mice were implanted with 5 × 105 B16-F10 cells and received PBS, AIL, IgG isotype control (IgG2a) or PD-L1 mAb treatment (n = 6). A Schematic of the treatment plan. B Photographs of tumor samples isolated from C57BL/6 mice receiving the indicated treatments. C Tumor growth curves for tumors. D Representative flow cytometric plots and quantification of CD25 + FOXP3 + Tregs in CD4 + cells after the indicated treatments. E Representative flow cytometric plots and quantification of CD4 + GZMB + in CD4 + T cells after the indicated treatments. F Representative flow cytometric plots and quantification of CD4 + IFNγ + in CD4 + T cells after the indicated treatments. G Representative flow cytometric plots and quantification of CD8 + GZMB + in CD8 + T cells after the indicated treatments. H Representative flow cytometric plots and quantification of CD8 + IFNγ + in CD8 + T cells after the indicated treatments. I Representative flow cytometric plots and quantification of PD-L1 + in CD45- cells after the indicated treatments. Data are shown as the mean ± SEM, n = 5; ns, not significant; *p < 0.05, **p < 0.01 and ***p < 0.001

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