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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Downregulation of Linc00173 increases BCL2 mRNA stability via the miR-1275/PROCA1/ZFP36L2 axis and induces acquired cisplatin resistance of lung adenocarcinoma

Fig. 2

LINC00173 inhibition attenuates the chemotherapeutic sensitivity of LUAD cells to DDP in vitro and in vivo. a. Colony formation assays were conducted to determine the colony numbers of LINC00173 downregulated A549 and PC9 cells with cisplatin treatment. Student’s two-tailed t-test, **P < 0.01; ***P < 0.001. b, c and d. CCK8 assays were performed to detect the cell growth (b), IC50 values (c) and cell viability (d) of LINC00173 silenced A549 and PC9 cells with cisplatin treatment. Student’s two-tailed t-test, **P < 0.01; ***P < 0.001; ****P < 0.0001. e. Fluorescence images of LINC00173 downregulated cells (shLINC00173-1/2) and their control parental cells (A549/PC9) before or after cisplatin treatment (10 μM). shLINC00173-1/2 or control shRNAs were labeled with GFP; Parental A549/PC9 cells were labeled with DsRed. Figures i, ii, iii and vii, viii, ix exhibit the mixture of an equal number of DsRed and GFP cells. Figures iv, v, vi and x, xi, xii represent the corresponding cisplatin treated cells (scale bar, 100 μm). One-way analysis of variance, ****P < 0.0001; ns, no significance. f. The effect of LINC00173 downregulation on cisplatin-induced apoptosis of A549 and PC9 cells detected by flow cytometry. Student’s two-tailed t-test, **P < 0.01. g. Expression levels of PARP, cleaved-PARP (c-PARP), Caspase3, cleaved-Caspase3, and BCL2 were determined by western blot in LINC00173-interfered A549 and PC9 cells which cultured with medium containing cisplatin. β-Tubulin was used as a loading control. h, i and j. Effect of LINC00173 on LUAD chemosensitivity to cisplatin in vivo. Growth curves (i) and weight of tumor xenografts (j) generated from A549 cells expressing either shRNAs targeting LINC00173 (shLINC00173-1) or nontargeting shRNA (scramble) and treated with cisplatin or control normal saline (N.S) (n = 5 for each group). Student’s two-tailed t-test, *P < 0.05; ** P < 0.01; ns, no significance. Error bars, mean ± SD

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