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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Downregulation of Linc00173 increases BCL2 mRNA stability via the miR-1275/PROCA1/ZFP36L2 axis and induces acquired cisplatin resistance of lung adenocarcinoma

Fig. 3

LINC00173 acts as a competing endogenous RNA to regulate PROCA1 by sponging miR-1275. a. Weighted gene co-expression network analysis (WGCNA) showed the closely relevant genes with LINC00173, including PROCA1. b. The effect of stable knockdown of LINC00173 on the expression level of candidate target genes verified by qRT-PCR in A549 and PC9 cells. Student’s two-tailed t-test, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. c. The correlation between LINC00173 and candidate gene PROCA1 by bioinformatics analysis (starBase_v3.0). d. miR-1275 mimics or negative control (NC) was co-transfected with LINC00173-WT or MUT in A549-DDP/PC9-DDP cells. Luciferase activity was measured by luciferase assay. Student’s two-tailed t-test, **P < 0.01; ***P < 0.001; ns, no significance. e and f. RNA antisense purification (RAP) assay and agarose gel electrophoresis confirmed the binding of miR-1275 with LINC00173 in A549/PC9 cells. Student’s two-tailed t-test, ****P < 0.0001. g and h. The effect of miR-1275 on the protein expression of PROCA1 was verified by Western blot. GAPDH was used as a loading control. i. Luciferase reporter assay was used to determine miR-1275 directly targets the 3′-UTR of PROCA1. Student’s two-tailed t-test, **P < 0.01; ***P < 0.001; ****P < 0.0001. Error bars, mean ± SD

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