Fig. 4
miR-1275/PROCA1 axis is essential for LINC00173-induced chemosensitivity to DDP in LUAD. a. miR-1275 inhibitors or negative control was transfected into LINC00173 stably silenced cells and cultured with cisplatin. Colony formation assay was performed to determine the effect of miR-1275 inhibition on the colony number of LINC00173 downregulated A549 and PC9 cells. Student’s two-tailed t-test, ***P < 0.001, ****P < 0.0001. b. miR-1275 inhibitors or negative control was co-transfected with LINC00173 smart silencer and cultured with cisplatin. Apoptosis of A549 and PC9 cells were measured by flow cytometry. Student’s two-tailed t-test, **P < 0.01; ***P < 0.001; ****P<0.0001. c-d. Overexpression of PROCA1 inhibited the chemoresistance of A549-shLINC00173-1 and PC9-shLINC00173-1 cells to cisplatin as examined by colony formation assay (c) and IC50 value (d). Student’s two-tailed t-test, **P < 0.01; ***P < 0.001. e–f. Co-transfection of PROCA1 with miR-1275 mimics inhibited the chemoresistance of A549 and PC9 cells to cisplatin as measured by colony formation assay (e) and IC50 value (f). Student’s two-tailed t-test, ***P < 0.001, ****P < 0.0001. Error bars, mean ± SD