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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Cyclin G2 in macrophages triggers CTL-mediated antitumor immunity and antiangiogenesis via interferon-gamma

Fig. 2

Depletion of cyclin G2 in macrophages reduces the chemotactic capacity of CTLs after IFN-γ treatment. A Representative CD8a immunohistochemistry staining of LLC tumors isolated from mice in the WT and Ccng2−/− groups. 10× scale bar = 200 μm; 40× scale bar = 50 μm. B Graph of the number of CD8-positive cells in each field (n = 5). Data are presented as the mean ± SEM and were analyzed using the unpaired Student’s t-test. C The number of CD8+T cells of LLC tumors isolated from mice in the WT and Ccng2−/− groups was evaluated by flow cytometry. D Data are presented as the mean ± SEM and were analyzed using the unpaired Student’s t-test (n = 3). E Schematic representation of the CTL chemotaxis assay. F Flow cytometric identification diagram for the CTLs. G, H Chemotaxis assays for CTLs treated with conditioned medium from BMDMs isolated from WT and Ccng2−/− C57BL/6 mice. Scale bar = 200 μm. The data were compared to the untreated (UT) groups using the unpaired Student’s t-test. Data are presented as the mean ± SD (representing 3 independent experiments). I The expression levels of granzyme B and perforin in the CTLs after treatment with conditioned medium from BMDMs isolated from WT and Ccng2−/− C57BL/6 mice were evaluated by flow cytometry. **p < 0.01; ****p < 0.0001

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