Fig. 6
Cyclin G2 promotes STAT1 nuclear translocation via PP2Ac. A Western blotting of whole cell lysates (WCL) from THP-1 stable cell lines (Vector and Flag-cyclin G2) followed by IP with anti-Flag. β-Tubulin was used as a loading control. B Cell lysates from THP-1 cells were immunoprecipitated with STAT1 or IgG control antibody, followed by western blotting with a PP2Ac antibody. β-tubulin was used as a loading control. C Cell lysates from THP-1 stable cell lines (Nonsense and shcyclin G2#1) were immunoprecipitated with STAT1 or IgG control antibody, followed by western blot analysis with PP2Ac antibody. D STAT1 and p-STAT1 (Y701) protein levels were detected in THP-1 cell lines (shcyclin G2#1-NC and shcyclin G2#1-siPP2Ac) by western blotting. β-tubulin was used as a loading control. E siNC and siPP2Ac were transfected into THP-1 (shcyclin G2) cells respectively, and the nuclear content of STAT1 was detected by immunofluorescence, representative images were shown. Scale bar = 10 μm. F STAT1 protein expression in the cytoplasm and nucleus of THP-1 cell lines (shcyclin G2#1-NC and shcyclin G2#1-siPP2Ac) was detected by western blotting. β-actin was used as a cytoplasmic loading control. Lamin B1 was used as a nuclear loading control