Fig. 6From: Sushi domain-containing protein 4 binds to epithelial growth factor receptor and initiates autophagy in an EGFR phosphorylation independent mannerSUSD4 colocalization with endo−/lysosomal markers. Representative merged confocal images showing EGFR (red), SUSD4 (green), endo−/lysosomal markers (blue) and DAPI (magenta) in BT-20 cells (A) or MDA-MB-468 cells (B) expressing SUSD4 and corresponding mock control cells. Note that the combination of the colors red, green, and blue is presented in white color. Colocalization coefficient for EGFR or SUSD4 with endo−/lysosomal markers quantified in BT-20 cells (C) and MDA-MB-468 cells (D) (Every data point represents the average of at least 10 cells per sample). All results were repeated in at least three independent biological experiments. Pearson’s correlation coefficient r where a minimal r value of 0.5 (dotted line) indicates significant colocalizationBack to article page