Fig. 4
A GSEA enrichment analysis of FASN-related genes based on the TCGA database. B Proliferation of sorafenib-exposed FASN-knockdown and control Huh7SR cells treated with different cell death inhibitors as determined with the CCK-8 assay. C and D Detection of lipid ROS levels in FASN-knockdown and control Huh7SR and 7721SR cells exposed to sorafenib as determined by flow cytometry. E MDA levels in FASN-knockdown and control Huh7SR and 7721SR cells exposed to sorafenib. F Expression levels of ferroptosis-related genes in FASN-knockdown and control Huh7SR and 7721SR cells as determined by RT-qPCR analysis. G and H SLC7A11 protein expression in FASN-knockdown and control Huh7SR and 7721SR cells. I and J Effect of SLC7A11 overexpression on lipid peroxidation levels in FASN-knockdown and control Huh7SR and 7721SR cells exposed to sorafenib. K Effect of SLC7A11 overexpression on MDA concentrations in FASN-knockdown and control Huh7SR and 7721SR cells exposed to sorafenib. *p < 0.05; **p < 0.01; ***p < 0.001. sora, sorafenib; Z-VAD, Z-VAD-FMK; Ferro1, ferrostatin-1; Necro-1, necrostatin-1