Fig. 6

KLF4 binds to the promoter of LAMA4. A Schematic illustration of pGL3-based reporter constructs used in luciferase assays to examine the transcriptional activity of LAMA4 named P1, P2 and P3. B P1, P2 and P3 together with the Renilla luciferase plasmid were transfected into 293 T cells with or without KLF4 expression. The Renilla luciferase construct was used to control for transfection efficiency, and dual luciferase activity was measured. C-D P2 together with the Renilla luciferase plasmid was transfected into MNNG/HOS and 143B cells with or without KLF4 knockdown for 24 h. Dual luciferase activity was measured. E P2 together with the Renilla luciferase plasmid was transfected into MNNG/HOS and 143B cells with or without LINC00629 knockdown for 24 h, and the cells were collected. Dual luciferase activity was measured. F P2 together with the Renilla luciferase plasmid was transfected into MNNG/HOS cells with or without KLF4 or LINC00629 knockdown for 12 h, and the cells were then treated with 3 μM TM for another 24 h. Dual luciferase activity was measured. G LINC00629 was overexpressed in MNNG/HOS cells with or without KLF4 knockdown, and P2 together with the Renilla luciferase plasmid was transfected into the cells for 24 h. Dual luciferase activity was measured. H Schematic illustration of the KLF4 wild-type binding site (BS) and the matching mutant (BSM) that was used in luciferase assays. I The wild-type promoter (BS) or the matching mutant (BSM) together with the Renilla luciferase plasmid was individually transfected into MNNG/HOS cells with or without KLF4 overexpression. Dual luciferase activity was measured. J The wild-type promoter (BS) or the matching mutant (BSM) together with the Renilla luciferase plasmid was individually transfected into MNNG/HOS cells with or without KLF4 knockdown. Dual luciferase activity was measured. K-L ChIP analysis showed the binding of KLF4 to the promoter of LAMA4 in MNNG/HOS cells with or without LINC00629 overexpression. Isotype-matched IgG was used as a negative control. Data in B, C, D, E, F, G, I, J were analysed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001