Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: CBL0137 impairs homologous recombination repair and sensitizes high-grade serous ovarian carcinoma to PARP inhibitors

Fig. 1

CBL0137 inhibits proliferation of HGSC cell lines and PDX lines. A, B SSRP1 protein expression was evaluated by Western blotting in the panel of HGSC cell lines (A) and patient-derived tumour cells (B). Actin was used as loading control. C, D A panel of HGSC cell lines (C) and patient-derived tumor cells (D) (blue – SSRP1-high, orange – SSRP1-low, and green – non-cancerous ovarian epithelial cells (OEC)) were treated with CBL0137 (0-2.5 μM) for 6 days. Cell viability was assessed using MTS assay (n = 3). The dose response curve was generated by GraphPad Prism 8. IC50 values of CBL0137 on a panel of HGSC cell lines (C) and patient-derived tumor cells (D). The data represented as mean IC50 value of three biological replicates. E OVCAR-8, OVCAR-4, PEO1, and PEO4 cells were treated with CBL0137 (0-2.5 μM) for 48 h and apoptosis was measured by Annexin V FITC/PI flow cytometry analysis. One-way ANOVA analysis was employed for statistical significance (n = 3). F OVCAR-8 cells were transfected with scramble (siNC) or SSRP1-specific siRNA for 24 h, followed by the treatment with 1 μM CBL0137 for 48 h. Left panel: Cell viability of OVCAR-8 cells, where unpaired Student’s t-tests were employed for statistical significance. Right panel: Representative Immunoblot for OVCAR-8 cells shows knockdown efficiency of SSRP1. Unpaired Student’s t-tests were employed for statistical significance. Representative Immunoblot shows knockdown efficiency of SSRP1

Back to article page