Fig. 2

BETi provoke metabolic reprogramming lowering intracellular LDs content. A Representative images of MDA-MB231 and Hs578t cells stained with ORO staining after 24 hours of 1 μM BETi treatment. B Representative images of MDA-MB231 cells stained with BODIPY 500/510 growth in normal condition (10% FBS, above) and in overnight starvation (1% FBS, below) after 24 hours of 1 μM BETi treatment. C Flow cytometry analysis of LDs content stained with BODIPY 500/510 in BC cell lines after 24 hours of 1 μM BETi treatment (MFI = median fluorescence intensity). D NMR analysis of neutral lipid content in MDA-MB231 cells after 24 hours of 1 μM BETi treatment