Fig. 5

Protein tyrosine phosphatase receptor R (PTPRR) is a target of miR-218-5p. A A schematic diagram for screening Parvimonas micra-regulated microRNAs and target genes. B qPCR detection of the PTPRR in the cells after coincubation with Pm-42, DH5α, or PBS. C Western blot detection of PTPRR in the cells after coincubation with Pm-42, DH5α, or PBS. D The mimics, inhibitor, or negative control (NC) of miR-218-5p were transfected into LoVo cells and coincubated with Pm-42, DH5α, or PBS. Then PTPRR was detected through qPCR. E The binding sites of miR-218-5p and PTPRR were predicted using TargetScan; dual luciferase plasmids and mutants containing PTPRR binding sequences were constructed. F miR-218-5p binding to PTPRR was detected using the luciferase reporter gene assay. G–H pcDNA3.1(+)-hPTPRR or pcDNA3.1(+) vectors were transfected into LoVo or HT-29 cells, then coincubated with bacteria; the MTT assay was used to detect cell proliferation. Data are expressed as mean ± SD from 3 independent experiments