Fig. 2

P300/CBP-mediated H3K27ac activates STRIP2 transcription in NSCLC. a Data from the UCSC genome bioinformatics site (http://genome.ucsc.edu/) showed high enrichment of H3K27ac in the promoter of STRIP2. b RT-qPCR analysis of STRIP2 mRNA level in C646 (5 μM)-treated A549 and PC9 cells at the indicated time point. c STRIP2 protein levels were measured by western blotting after C646 (5 μM)-treated A549 and PC9 cells for 24 hours. d The efficiency of P300 and CBP knockdown was performed using RT-qPCR. e The P300, H3K27ac and STRIP2 levels were detected using western blotting after P300 or CBP knockdown. f CHIP assay were used to measure the level of P300 or CBP binding at the promoter of STRIP2 in P300 or CBP deficiency or control A549 cells. Data represent mean ± SEM from three independent experiments. *, p < 0.05; **, p < 0.01 were determined by one-way ANOVA with Tukey’s post hoc analysis. H3K27ac, H3K27 acetylation; CHIP, chromatin immunoprecipitation