Fig. 9

STRIP2 cooperates with IGF2BP3 to stabilize TMBIM6 mRNA via m6A. a Data from the RMVar bioinformatics site (https://rmvar.renlab.org/) showed exists m6A site in the 3′-UTR of TMBIM6. b-c RIP-PCR analysis detecting the enrichment of TMBIM6 m6A modifications. d-g RT-PCR analysis the mRNA of TMBIM6. h Construction of TMBIM6-WT and Mut luciferase reporter plasmids. i-l Dual-luciferase activity in cells co-transfected with TMBIM6-WT or TMBIM6-Mut together with STRIP2 or IGF2BP3 overexpression and knockdown was measured. Data represent Mean ± SEM from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 were determined by one-way ANOVA with Tukey’s post hoc analysis. OE, overexpression