Fig. 3

Knockdown of ENO2 inhibits growth and glucose metabolism of HNSCC cells through downregulating PKM2. a, b Effect of ENO2 knockdown (a) and overexpression (b) on glycolytic molecules determined by western blotting. c Restoration of PKM2 expression in ENO2 knockdown UM-1 and Cal27 cells. d-f Effect of PKM2 expression restoration on cell proliferation (d) and intracellular levels of ATP (e) and glucose (f) in ENO2 knockdown UM-1 and Cal27 cells. g Effect of 5µM compound 3k (Com 3k) on cell proliferation in ENO2 overexpressing (ENO2 O/E) and control Cal33 and HSC3 cells. h Effect of 5µM compound 3k on intracellular levels of ATP in ENO2 overexpressing and control Cal33 and HSC3 cells. i Effect of 5µM compound 3k on intracellular levels of glucose in ENO2 overexpressing and control Cal33 and HSC3 cells.  *p<0.05; **p<0.01