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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: RNF31 represses cell progression and immune evasion via YAP/PD-L1 suppression in triple negative breast Cancer

Fig. 1

RNF31 restrains cancer cell progression in TNBC (A) Experimental scheme to test cell biological function. (B-D) Western blot detecting of RNF31 expression in MDAMB231 and BT549 cells exposed to indicated methods. (E-F and O) Transwell assay (left panel) of MDAMB231(E) and BT549(F) cells. Right panel shows quantification of transwell assay results. Scale bar 100 μm. (G-H and Q) FACS analysis (left panel) was performed on the MDAMB231 and BT549 cells to detect the proportion of CD44 + CD24-cells. Right panel shows quantification of CD44 + CD24- proportion. (I-J and R) FACS analysis (left panel) was performed on the MDAMB231and BT549 cells to detect the proportion of apoptotic cells. The cells were incubated with PI and Annexin V. Right panel shows quantification of apoptosis proportion. (K-L) Wound healing assay (left panel) of MDAMB231(K) and BT549(L) cells migration capability following transfected with indicated treatment. Quantification of wound closure at the specified points in time. Right panel shows quantification of wound healing results. (M-N and P) Representative images (left panel) of EdU assays in MDAMB231and BT549 cells transfected with indicated treatment. EdU-positive cells, red; cell nuclei, blue. Right panel shows quantification of Edu results. Scale bar 100 μm. (S) Representative image of tumor derived from BALB/c nude mice injected with Myc or Myc-RNF31 stably transfected MDAMB231 cells is followed as indicated. (T-U) The tumor volume (T) and weight (U) in BALB/c nude mice subcutaneously inoculated Myc or Myc-RNF31 stably transfected MDAMB231 cells. (V) Western blot detecting of RNF31 expression in tumor tissues in nude mice injected with Myc or Myc-RNF31 stably transfected MDAMB231 cells. The results are representative of 3 independent experiments in panel B-R. The results are representative of 5 independent experiments in panel S-U. β-actin was engineered to the internal reference for Western blot. The data are presented as mean ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test)

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