Fig. 4

Cell viability and sensitivity to HER2-targeting treatment after silencing EDI3 using siRNA. Viability relative to negative control (si-NEG) after silencing EDI3 with three siRNAs targeting different exons in A, SKBR3. B, HCC1954, C, BT474 and D, EFM192A cells. Viability presented as RFU after silencing EDI3 using siRNA (oligo #1) with and without lapatinib (0.01, 0.1 and 1 µM) for 96 h in E, SKBR3, F, HCC1954, G, BT474 and H, EFM192 cells. Data are mean ± SD (A-D) and mean ± SE (E–H) of at least three independent experiments (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant). RFU, relative fluorescence units