Fig. 6

M6A regulation of LATS1 mRNA was identified by ythdf2 in breast cancer cells. a Deletion of METTL3 improved the stability of LATS1 mRNA, n = 3, * p < 0.05, *** p < 0.001. b Conducting the m6A methylation sites mutation on LATS1 mRNA helped enhance the stability of LATS1 mRNA, n = 3, * p < 0.05. c RNA-pull down assay showed the interaction between YTHDF2 and LATS1 mRNA. d Altering the expression of METTL3 and mutating the LATS1 mRNA binding site of METTL3 affected only the protein level of LATS1. e YTHDF2 negatively regulated the protein level of LATS1 in breast cancer cells. f Rescue of the expression of LATS1 by altering the protein level of METTL3 together with YTHDF2 affected the phosphorylation level of YAP/TAZ in the nucleus