Fig. 3

Secretome analysis reveals differences and overlaps with normal lung fibroblasts and CAFs from colon, lung and breast cancer. A Proteins in the supernatant fractions of Meso-CAFs and normal lung fibroblasts (NLF) were subjected to unsupervised clustering. B Selected secreted proteins with differential expression between Meso-CAFs and NLFs. Values are shown as mean and SEM of 3 biological replicates performed in duplicates. C Proteins only identified in Meso-CAFs or significantly upregulated in Meso-CAFs compared to NLFs were subjected to Gene Ontology (GO) analysis and the 6 most significantly associated biological processes are shown. D Venn diagram showing the overlaps in secreted proteins identified in CAFs from the indicated cancer types. E Heatmap of Jaccard similarity coefficients indicating the degree of secretome overlap between CAFs from the indicated cancer types