Fig. 5

Crizotinib, LY294002 and WNT-C59 block the growth stimulation of PM cells by Meso-CAFs. Green fluorescent protein tagged (GFP⁺) SPC212 cells (A) and MSTO-211H (B) were incubated without conditioned medium (−CM) or with CM conditioned by Meso109F (+109F-CM) or Meso125F (+125F-CM). Micrographs were taken every 5 h and numbers of GFP⁺ tumor cells (TC) were determined by automated image analysis. * p < 0.05, ** p < 0.01, *** p < 0.001 TC number in the presence versus absence of CM, two-way ANOVA with Tukey’s multiple comparisons test. GFP⁺ SPC212 (C) or MSTO-211H (D) were cultured as monolayers in the absence of CAFs (−CAF) or in the presence of Meso109F (+109F) or Meso125F (+125F) and treated with crizotinib, erdafitinib, erlotinib, galunisertib, LY294002, WNT-C59 or vehicle (DMSO). Numbers of GFP⁺ TC after 72 h were calculated by automated image analysis. ** p < 0.01, *** p < 0.001 inhibitor treated versus vehicle treated, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 growth in presence of Meso-CAFs versus growth in absence of Meso-CAFs, ° p < 0.05, °° p < 0.01, °°° p < 0.001 percent inhibition in presence of Meso-CAFs versus percent inhibition in absence of Meso-CAFs, one-way ANOVA with Tukey’s multiple comparisons test