Fig. 2

TRIM21 silencing enhances lipid accumulation in RCC cells. A-B Representative images of Oil Red O staining when TRIM21 was knocked down or overexpressed in ACHN, 786-O, and Caki-1 cells (red arrows indicate lipid droplets). C Nile red staining and fluorescence microscopy were performed when TRIM21 was knocked down in ACHN, 786-O, and Caki-1 cells. The red fluorescence intensity of labeled lipid droplets increased (red fluorescence indicates lipid droplets, blue fluorescence DAPI staining nuclear). D Nile red staining and fluorescence microscopy were performed when TRIM21 was over-expressed in ACHN, 786-O, and Caki-1 cells. The red fluorescence intensity of labeled lipid droplets decreased (red fluorescence indicates lipid droplets, blue fluorescence DAPI staining nuclear). E Flow cytometry was used to access the fluorescence intensity of Nile red staining. The fluorescence intensity of labeled lipid droplets was enhanced when TRIM21 was knocked down. F The fluorescence intensity of labeled lipid droplets was decreased when TRIM21 was over-expressed. All the results were confirmed by three times repeated experiments