Fig. 3

TRIM21 overexpression significantly decreases lipid contents in RCC cells. A Western blot was used to detect the stable overexpression efficiency of TRIM21 in ACHN. GAPDH was used as a loading control. B The number of lipid subclasses and lipid molecules was detected by mass spectrometry. C The volcano plot shows the overall differential expression of lipid molecules. The rose-red dots show the differential lipid molecules screened by univariate statistical analysis. D The hierarchical clustering results of different lipids in the control group (Vector) and TRIM21 overexpression group (TRIM21). The horizontal coordinate represents the group of samples, and the vertical coordinate represents the different lipid molecules. The results showed that the expression levels of various lipid molecules in the TRIM21 group were significantly lower than those in the Vector group. The relative contents of (E) triglyceride (F) diglycerol and (G) cholesterol in the OE-TRIM21 group were significantly lower than those in the Vector group. *p < 0.05, **p < 0.01