Fig. 5

SREBF1 is critical for TRIM21-mediated lipogenesis inhibition in vitro. A and B Western blot was used to detect the stable knockdown and overexpressed efficiency of TRIM21 and SREBF1 alone or combination with in ACHN, and GAPDH was used as a loading control. Representative images of Oil Red O staining when TRIM21 and SREBF1 were stably knocked-down (C) or over-expressed (D) alone or combination with in ACHN cells (red arrows indicate lipid droplets). Flow cytometry (E) and a microplate reader (G) were used to access the fluorescence intensity of Nile red staining when TRIM21 and SREBF1 were stably knocked down alone or simultaneously. Flow cytometry (F) and a microplate reader (H) were used to access the fluorescence intensity of Nile red staining when TRIM21 and SREBF1 were stably overexpressed alone or simultaneously. All the results were confirmed by three times repeated experiments. Data are presented as the means ± SEM for experiments in triplicate. ns, no significance, **p < 0.01, ***p < 0.001