Fig. 6
From: TRIM21 attenuates renal carcinoma lipogenesis and malignancy by regulating SREBF1 protein stability
TRIM21 inhibits RCC tumorigenesis by modulating SREBF1-regulated lipid accumulation in an orthotopic model. NSG mice were randomly divided into a control group (Vector), TRIM21 overexpression group (OE-TRIM21), SREBF1 overexpression group (OE-SREBF1), and TRIM21 + SREBF1 double overexpression group (OE-TRIM21 + OE-SREBF1). The cell suspension (5 × 105cells/10μL) was injected under the renal capsule. Representative in vivo imaging images of each group was shown in (A), and the fluorescence intensity of each group was counted in B. C Representative images of Oil Red O staining of renal orthotopic tumor in each group. D-E IHC detected Ki-67 expression and quantification of Ki-67-positive cells in tumors. E Representative examples of IHC-based correlation between TRIM21 and SREBF1 expression in the renal orthotopic tumor. Statistical analysis was performed using unpaired t-tests. All statistical tests were two-sided. **p < 0.01, ***p < 0.001