Fig. 7
From: TRIM21 attenuates renal carcinoma lipogenesis and malignancy by regulating SREBF1 protein stability
TRIM21 is negatively correlated with SREBF1 in RCC patients and TRIM21-SREBF1 is a reliable combinational biomarker for RCC prognosis. A The Riskscore, survival time, and survival status of the RCC dataset. The top scatterplot represents the Riskscore from low to high. Different colors represent different groups. The scatter plot distribution represents the Riskscore of different samples corresponding to the survival time and survival status. The bottom heatmap is the SREBF1 and TRIM21 expression from the signature. B Kaplan–Meier survival analysis of the risk model from the RCC dataset, comparison among different groups was made by log-rank test. HR (High groups) represents the hazard ratio of the low-expression sample relatives to the high-expression sample. HR > 1 indicates that TRIM21 and SREBF1 are combinational risk factors. HR (95%Cl), the median survival time (LT50) for different groups, in years. C The ROC curve and AUC of the TRIM21 and SREBF1. The higher values of AUC correspond to higher predictive power. D-E Representative immunohistochemistry images of TRIM21 and SREBF1 protein expression in carcinoma tissues in RCC patients. F-G Analyzing the relevant SREBF1 expression in TRIM21-low cases and TRIM21-high cases. H The correlation between TRIM21 and SREBF1 expression was analyzed in RCC tissues according to the scores of immunohistochemistry staining by Pearson’s correlation coefficient test. I Overall survival rates were analyzed between four subgroups (TRIM21high/SREBF1low, TRIM21high/SREBF1high, TRIM21low/SREBF1low, TRIM21low/SREBF1high). J A cartoon summarizing our findings. TRIM21 binds to SREBF1, a transcription factor that has a central role in lipogenesis, and mediates the ubiquitination degradation of SREBF1, leading to the decreased transcriptional expression of downstream lipid metabolism-related genes, and therefore fine-tunes the lipid metabolism process of RCC. Statistical analysis was performed using unpaired t-tests. All statistical tests were two-sided. **p < 0.01, ***p < 0.001