Fig. 1

ac4C mRNA acetylation and NAT10 expression are upregulated in gastric cancer. A The ac4C/C ratio in polyA( +) mRNA isolated from 20 GCs (tumor) and paired normal gastric mucosal tissues (Normal) was quantified by HPLC–MS/MS. B The expression levels of NAT10 in GC vs. nontumor gastric tissues from the TCGA data and GEO datasets. C qRT–PCR analysis of NAT10 mRNA levels in 20 pairs of GC and adjacent normal tissues. A-C P values were calculated using a two-tailed t-test. D Pearson correlation analysis showing that the ac4C acetylation level is correlated with the mRNA expression of NAT10 in GC specimens. E Western blot assay of NAT10 protein levels in 15 GC tissues and paired normal tissues. T, gastric tumor; N, adjacent normal tissue. F IHC analyses of NAT10 expression in 202 GC and 185 adjacent normal tissues on tissue microarrays. Scale bar: 150 μm. G Overall survival curves for GC patients in the TMA cohort with high or low expression of NAT10. H Kaplan–Meier analyses of OS, FP and PPS for GC patients based on NAT10 expression using the online tool Kaplan–Meier Plotter. I Multivariate Cox analysis of factors associated with OS in TMA samples. CI, confidence interval; HR, hazard rate. Error bars, SD