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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Helicobacter pylori-induced NAT10 stabilizes MDM2 mRNA via RNA acetylation to facilitate gastric cancer progression

Fig. 5

NAT10 overexpression correlates with high levels of MDM2 ac4C modification and MDM2 expression in gastric cancer specimens. A and B The ac4C levels of the MDM2 transcript were measured by acRIP-qPCR analysis (A), and MDM2 mRNA levels were tested by qRT–PCR (B) in 20 GC and paired normal gastric mucosal tissues. The differences were determined with a two-tailed t-test. C ac4C levels of MDM2 mRNA were positively correlated with MDM2 and NAT10 expression in GC specimens. D The graph shows a significant correlation of NAT10 mRNA with MDM2 expression in GCs. E The TCGA and GEO datasets shows that NAT10 and MDM2 levels were correlated in GC tissues. r and P values were determined by Pearson correlation test (C-E). FH Representative images of IHC staining of NAT10 and MDM2 in normal gastric tissues and two GC samples with high or low expression of both proteins are shown (F). Scale bar, 150 μm. NAT10 expression was positively correlated with MDM2 expression (G). r and P values were calculated using the Pearson correlation test. Kaplan–Meier analysis indicates the correlation between the combination of high expression of NAT10 and MDM2 and poorer OS (H). Error bars, SD

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