Fig. 6

Hp infection enhances NAT10 expression and regulates p53 stability. A Western blot analysis of p53, NAT10 and MDM2 following coculture of GES1 and AGS cells with Hp. B The stability of p53 protein was determined in GES1 cells cocultured with Hp SS1 using the cycloheximide chase method. C qRT–PCR was performed to analyze NAT10, MDM2 and CDKN1A expression in the indicated cells. D and E qRT–PCR analysis of NAT10, MDM2 and CDKN1A expression (D) and Western blot analysis of NAT10, MDM2 and p53 (E) in gastric tissues from mice challenged with Hp SS1 or Brucella broth (Control) for 3 weeks. F The global ac4C acetylation levels in mRNA and total RNA from GES1 and AGS cells cultured in the presence or absence of Hp. G The ac4C levels of MDM2 mRNA in cells treated as described in H. H The stability of MDM2 mRNA was assessed in the indicated cells treated with 5 μg/mL ACD. The MDM2 mRNA abundance relative to that of GAPDH as quantified by qRT–PCR. I Protein levels were tested in NAT10-knockout and control cells following coculture with Hp. SS1, Hp strain SS1; 43,504, Hp strain ATCC43504. Error bars, SD. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed t-test)